Avian influenza virus H7 antigen rapid test card ,4mm cassette
rapidly and easily, gold colloidal method
Intended Use
Avian influenza hemagglutinin according to their outer membrane (H)
and neuraminidase (N) protein antigen is divided into a plurality
of different and different subtypes. H7 avian influenza virus
antigen rapid test card is specifically designed to detect H7
subtypes of the virus detection reagent.
The AIV H7 Ag test strip is based on fast immuno-chromatography
technique to detect AIV H7 Ag in birds trachea or cloaca (or
feces). After adding sample, the sample move along with the
colloidal gold labeling H7-AIV antibody, is there is H7-AIV antigen
in the sample, it combines with antibody on T line and show
wine-red color; if there is no H7-AIV antigen, then no color
reaction.
Components
| 1 | H7 Ag test card | 50 pieces |
| 2 | Swab | 50 pieces |
| 3 | Dropper | 50 pieces |
| 4 | Sample buffer | 50 bottles |
| 5 | Disposable gloves | 10 pairs |
| 6 | Manual | 1 piece |
Test Procedure
1) Use Swab to collect sample from cloaca (recommended) or trachea,
the swab should be inserted into cloaca or trachea, wipe and rotate
in inner well repeatedly. To young birds, it can use dropper to
collect fresh feces directly.
2) Insert the swab into the tube with sample buffer immediately,
rotate the swab strongly on tube well for at least 10 times and mix
the solution evenly, making the sample been dissloved in sample
buffer fully. Press the swab on tube well above the liquid level,
making the liquid been pressed out fully, discard swab.
3) If the particles are big in feces, it may be static for 1-2 min,
making bigger particles precipitate.
4) Take out test card from sealed bag. Put it on flat surface, use
dropper to draw upper-layer bright liquid, drop 5 drops slowly, one
by one, to the sample hole with “S” mark.
5) Put the test card at room temperature for 10-15min, read the
result, the result is invalid after 15 minutes.
INTERPRETATION OF RESULTS
Positive: control line and test line are both seen wine red, the more the
antibody exist, the thicker the color appear;
2) Weak positive: control line and test line are both seen wine red, but the color
of test line is very light;
3) Negative: only control line is seen wine red;
4) Invalidation: control line isn’t seen wine red.
QUALITY CONTROL
A procedural control is included in the test. A line appearing in
the control region
(C) is considered an internal procedural control. It confirms
sufficient specimen volume, adequate membrane wicking and correct
procedural technique.
Control standards are not supplied with this kit. However, it is
recommended that positive and negative controls be tested as good
laboratory practice to confirm the test procedure and to verify
proper test performance.
Note
1) Do not use product with broken Aluminum foil bag package. Use
test card in 1 hour after taking out.
2) The collected sample should be tested immediately, avoid long
time storage.
3) The sample collection directly affect the accuracy of testing,
so ensure collect sample correctly and fully.
4) Collect feces sample too much will affect the normal surge of
liquid, and lead to wrong result (black area appear), and too
little sample may lead to false negative result, so collect sample
as following picture.
5) Avoid bubble when adding sample to test card.
6) The used test card should be handled as local pollution
properly.
7) Do not use test card out of expiry date.
8) Deal with the used test card and sample as waste according to
local regulation strictly.
Frequently Asked Questions
1) Limitation of the product
This kit is a qualitative screening reagent, can detect AIV antigen
accurately, but if sample is not enough or concentration of antigen
is lower than sensitivity, it may appear negative result. A clear
clinical diagnosis should be made after taking into account all
clinical and laboratory phenomenon.
2) The sensitivity and specificity of the product.
The limit value to detect AIV H7 Ag is 0.13 HAU, reaction with H1N1
/ H2N2 / H3N2 / H5N1 / H9N2 strains have a negative representative.
it has no cross-reaction with similar symptoms to those of fowl
cholera, newcastle disease, infectious bronchitis
| ORIENT NEW LIFE MEDICAL CO., LTD. |
| Contact: | Jerry Meng |
| Email: | Jerry @ newlifebiotest .com |
| Tel. | +86 18657312116 |
| SKYPE | enetjerry |
