HBeAg ELISA test detect Hepatitis B E antigen,96wells/kit, Elisa Sandwich method, for quantitative measurement

1. HBeAg EIA is a qualitative enzyme immunoassay for the detection of HBeAg
2. human serum or plasma.
3. in vitro diagnostic kit

SUMMARY

This kit uses “ sandwich” ELISA method in which, polystyrene microwell strips are pre-coated with monoclonal antibodies specific to HBeAg. Patient’s serum or plasma sample is added to the microwell together with a second monoclonal antibody conjugated to horseradish peroxidase (HRP-Conjugate).During incubation, the specific immunocomplex formed in case of presence of HBeAg in the sample is captured on the solid phase. After washing to remove sample and unbound HRP-Conjugate, Chromogen solutions containing Tetramethylbenzidine (TMB) and urea peroxide are added into the wells. In presence of the antibody-antigen-antibody(HRP) “sandwich “ complex, the colorless Chromogens are hydrolyzed by the bound HRP conjugate to a blue-colored product. The blue color turns yellow after stopping the reaction with sulfuric acid. The amount of color intensity can be measured and is proportional to the amount of antigen captured in the wells, and to the sample respectively. Wells containing samples negative for HBeAg remain colorless.

PRINCIPLE

This kit is an enzyme -linked immunosorbent assay (ELISA) for qualitative detection of hepatitis B virus e antigen (HBeAg) in human serum or plasma. It is intended for use in clinical laboratories for diagnosis and management of patients related to infection with hepatitis B virus. HEPATITIS B VIRUS E ANTIGEN ELISA. One-Step Incubation, Double Antibody Sandwich Principle

REAGENT

Materials provided with the kits:

1. Negative Control: 0.5ml HBeAg Negative Control.
2. Positive Control: 0.5ml HBeAg Positive Control.
3. Enzyme Conjugate:.6 ml containing HRP-conjugated-anti-HBe
4. Wash Buffer Concentrate (20 x): 20 ml for 96 tests. The buffer should be diluted 20 times with distilled water before use.
5. Substrate Solution A: 6 ml HRP Substrate for 96 tests.
6. Substrate Solution B: 6 ml TMB Chromagen Substrate for 96 tests.
7. Stop Solution: One bottle of 6 ml 2N Sulfuric Acid

Materials required but not provided:

1. Precision pipettes: 0.02, 0.05, 0.10, 0.15, 0.20, and 1.0 ml.
2. Disposable pipette tips.
3. Distilled water.
4. Humidified Box capable of maintaining 37°C
5. Absorbent paper or paper towel.
6. Micro titer plate or strip-well washer
7. Micro titer plate reader.

Established in 2009, Orient New Life Medical Co., Ltd., is specialized in high-tech biological diagnostic test development, research and production, as well as high-tech medical device development, research and production. New Life has built up two production base, which produce biological diagnostic test and endoscopic medical device respectively. Our products ranged in the following specialties: drug of abuse tests, fertility tests, infectious diseases tests, sexual transmitted diseases tests, tumor markers tests, cardiac markers tests, veterinary tests, and surgical stapler series.

NEW LIFE have 3 wholly-owned and joint stock subsidiary companies, including Orient New Life Medical  Co., Ltd., Orient Gene Biotech Co., Ltd., Qianjing Medical Equipment Co., Ltd.

Company's core technical team with more than 35 employees，3 doctors and 25 masters. They have many years of famous universities in the United States and pharmaceutical companies engaged in the project research and development, production and marketing, business management experience.Company owns hundreds of grade purification workshop and all level of quality checkout room, equipped with various production and testing equipment.