HIV 1/2 antibody elisa test kitt,96wells/kit, high accuracy Elisa
Sandwich method, for quantitative measurement
Materials provided with the kit:
| 96T |
| Coated Microtiter Plate | 1 bag | 12*8 |
| Conjugate | 1vial | 10.5mL |
| Biotin Working Liquid | 1vial | 3mL |
| Wash Buffer Concentrate (40*) | 1vial | 20mL |
| Substrate A | 1vial | 7mL |
| Substrate B | 1vial | 7mL |
| Stop Solution | 1vial | 6mL |
| Negative Control | 1vial | 1mL |
| Positive Control | 1vial | 1mL |
| Closure Plate Membrane | 3sheet | |
Note: different batches of reagent kit, and different component can
not be exchanged for use. Once open, stable for 3 months at 2-8°C.
INTENDED USE
The Anti-HIV 1+2 ELISA is a qualitative enzyme immunoassay for the
in vitro detection of Human
Immunodeficiency Virus (HIV) infection, based on the detection of
antibodies to HIV-1 and HIV-2 in human serum or plasma. It is
intended for screening of blood donors and diagnosing patients
related to infection with HIV.
ASSAY PROCEDURE
1. Prepare Reagents: Dilute 1 volume of Concentrated Washing Buffer
with 19 volumes of distilled water,
mix well.
2. Add Samples: Open the foil pouch and remove the Microplate. Set
up 1 well as Blank, 2 wells as negative control, 2 wells as
positive control. After dispensing 50ul of Sample Diluent ,
dispense 50 ul of sample or negative control or positive control to
the respective wells. Gently vibrating the plate.
3. Incubate: Cover the Microplate with plate cover and incubate the
Coated Microplate in a thermostat-controlled water-bath or
microplate incubator at 37 for 60 minutes.
4. Wash the Plate: Remove the plate cover. Aspirate the contents of
all wells. Fill the wells with the diluted
washing buffer ( 10~20 seconds to soak) then aspirate again. Repeat
the procedure for 5 times. Make sure that the rest volume is
minimal, by tapping plate onto absorbent paper.
5. Add Conjugate: Add 100ul of conjugate to each well (except the
blank well).
6. Incubate: Cover the Microplate and incubate the plate at 37 for
30 minutes.
7. Wash the Plate: Repeat the wash procedure as in step 4.
8. Add Substrate: Add 50ul of Substrate Solution A and 50 ul of
Substrate Solution B to each well, mix well.
9. Stop reaction: Add 50ul Stop Solution to each well, mix well.
10. Read the absorbance at 450 nm. If a dual wavelength measurement
is used, the reference wavelength should
| Name | HIV 1,2 ELISA Test |
| Full name | Human Immunodeficiency Viruses Type 1,2 Antibody (HIV1,2) ELISA
Test Kit |
| Category Name | HIV ELISA kit |
| Test | 96 Tests |
| Method | ELISA: Enzyme Linked Immunosorbent Assay |
| Principle | ELISA: Sandwich; Double antigen |
| Detection Range | Qualitative: Positive, negative and cutoff calibrators |
| Sample | 100 uL Serum or plasma |
| Specificity | 99.89% |
| Sensitivity | 99.95% |
| Total Time | 15 Min |
| Shelf Life | 12 Months from the manufacturing date |
| ORIENT NEW LIFE MEDICAL CO., LTD. |
| Contact: | Jerry Meng |
| Email: | Jerry @ newlifebiotest .com |
| Tel. | +86 18657312116 |
| SKYPE | enetjerry
|
