high sensitivity Drug Abuse Test Kit ) GHB Rapid Test 3mm strip ,
cut-off 10 μg/mL
Product Name
GHB Rapid Diagnostic Test
Specimen: Urine
Background
γ-Hydroxybutyric acid (GHB), also known as 4-hydroxybutanoic acid,
is a naturally occurring neurotransmitter and a psychoactive drug.
It has been used in a medical setting as a general anesthetic and
as a treatment for cataplexy, narcolepsy, and alcoholism. It is
also used illegally as an intoxicant, to try to increase athletic
performance, and as a date rape drug. It is commonly used in the
form of a salt, such as sodium γ-hydroxybutyrate (Na.GHB, sodium
oxybate) or potassium γ-hydroxybutyrate (K.GHB, potassium oxybate).
Urine is often the preferred specimen for routine drug abuse
monitoring purposes. Both γ-butyrolactone (GBL) and 1,4-butanediol
are converted to GHB in the body. Other liquids such as suspected
spiked drinks can also be tested. However, cross-reactivity with
Ascorbic Acid and Alcohol need to be taken into account, while
interpreting the test results.
Test Principle
GHB-DH catalyses the reaction of GHB and NAD to produce NADH, and a
diaphorase couple tetrazoliym dye reaction results in the
production of a purple dye complex. The reagents were stabilized
and used to produce the test to screen for GHB in urine at 10
μg/mL.
SPECIMEN COLLECTION AND STORAGE
The urine specimen must be collected in a clean and dry container.
Urine collected at any time of the day may be used. Urine specimens
exhibiting visible particles should be centrifuged, filtered, or
allowed to settle to obtain clear specimen for testing. Specimen.
Urine specimens may be stored at 2-8°C for up to 48 hours prior to
testing. For long-term storage, specimens may be frozen and stored
below -20°C. Frozen specimens should be thawed and mixed before
testing.
STORAGE
Store as packaged in the sealed pouch either at room temperature or
refrigerated (2-30°C). The test is stable through the expiration
date printed on the sealed pouch. The test must remain in the
sealed pouch until use. DO NOT FREEZE. Do not use beyond the
expiration date.
LIMITATION
1. The 6-MAM Rapid Test Cassette provides only a qualitative,
preliminary analytical result. A secondary analytical method must
be used to obtain a confirmed result. Gas chromatography/mass
spectrophotometry (GC/MS) is the preferred confirmatory method2.3.
2. It is possible that technical or procedural errors, as well as
other interfering substances in the urine specimen may cause
erroneous results.
3. Adulterants, such as bleach and/or alum, in urine specimens may
produce erroneous results regardless of the analytical method used.
If adulteration is suspected, the test should be repeated with
another urine specimen.
4. A positive result indicates presence of the drug or its
metabolites but does not indicate level of intoxication,
administration route or concentration in urine.
5. A negative result may not necessarily indicate drug-free urine.
Negative results can be obtained when drug is present but below the
cut-off level of the test.
performance characteristics
A side-by-side comparison was conducted using the GHB Rapid Test
Cassette (Urine) and GC/MS. The following results were tabulated:
Table 1: Specimen Correlation
| Method | | | GC/MS | Total Results |
| GHB Rapid Test | | Results | Positive | | Negative | |
| Positive | 46 | | 4 | 50 |
| Cassette (Urine) | | |
| Negative | 4 | | 136 | 140 |
| | |
| Total Results | | | 50 | | 140 | 190 |
| % Agreement | | 92.0% | | 97.1% | 95.8% |
| ORIENT NEW LIFE MEDICAL CO., LTD. |
| Contact: | Jerry Meng |
| Email: | Jerry @ newlifebiotest .com |
| Tel. | +86 18657312116 |
| SKYPE | enetjerry |
