Recombinant Anti COVID 19 Mouse Monoclonal Antibodies Mab
Monoclonal Antibody
Recombinant anti COVID 19 Mouse monoclonal antibodies
Background
The spike (S) glycoprotein of coronaviruses contains protrusions
that will only bind to certain receptors on the host cell. Known
receptors bind S1 are ACE2, angiotensin-converting enzyme 2; DPP4,
dipeptidyl peptidase-4; APN, aminopeptidase N; CEACAM,
carcinoembryonic antigen-related cell adhesion molecule 1; Sia,
sialic acid; O-ac Sia, O-acetylated sialic acid. The spike is
essential for both host specificity and viral infectivity. The term
'peplomer' is typically used to refer to a grouping of heterologous
proteins on the virus surface that function together. The spike (S)
glycoprotein of coronaviruses is known to be essential in the
binding of the virus to the host cell at the advent of the
infection process. It's been reported that SARS-CoV-2 (COVID-19
coronavirus, 2019-nCoV) can infect the human respiratory epithelial
cells through interaction with the human ACE2 receptor. The spike
protein is a large type I transmembrane protein containing two
subunits, S1 and S2. S1 mainly contains a receptor binding domain
(RBD), which is responsible for recognizing the cell surface
receptor. S2 contains basic elements needed for the membrane
fusion. The S protein plays key parts in the induction of
neutralizing-antibody and T-cell responses, as well as protective
immunity.
| Name | Recombinant anti COVID 19 Mouse monoclonal antibody Recombinant SARS-CoV Spike S1 Subunit Protein | Application | Invitro research and manufacture use;
membrane assay, ELISA assay, |
| Formulation | 0.2 μm filtered solution in PBS | Buffer: | 1X PBS buffer |
| Storage: | This antibody can be stored at 2℃-8℃ for one month without
detectable loss of activity. Antibody products are stable for
twelve months from date of receipt when stored at -20℃ to -80℃.
Preservative- Free. Avoid repeated freeze-thaw cycles. | Concentration | ELISA: 1:5000-1:10000 |
| Expiration Date: | 30 days refrigerated at -2-8ºC
and twelve months frozen at -20 ºC
or below from production date.
To be evaluated independently | Feature | High Sensitivity and Specificity |
Specification Data
| Description: | Monoclonal Antibody to Anti COVID-19 |
| Clone: | 007 | |
| Source: | Rabbit IgG | |
| Immunogen: |
SARS-CoV-2 (2019-nCoV) Spike S1 Protein SARS-CoV-2 (2019-nCoV) Spike RBD Protein SARS-CoV Spike S1 Protein SARS-CoV Spike RBD Protein | |
| Formulation: |
0.2 μm filtered solution in PBS | |
| Purification: | DEAE chromatography | |
| Concentration: | 1.0 mg/mL (OD280 nm, E0.1% = 1.40) |
| Affinity Constant: | Not determined | |
| Buffer: | 0.015M potassium phosphate buffer, 0.85% NaCl, 0.05% NaN3 pH=7.2 |
| Pairing Assays: | N/A | |
| Purity: | >95% by HPLC and SDS-PAGE |
| Application: |
ELISA IHC, FCM, IF, IP et al. applications haven't been validated. |
Immunofluorescence staining of SARS-CoV-2 (2019-nCoV) Spike in
ACE2-overexpressed 293T cells, infected (left) or noninfected
(right) by 2019-nCOV-Spike pseudovirus (PSV). Cells were blocked
with 10% serum, and incubated with SARS-CoV-2 (2019-nCoV) Spike
rabbit monoclonal antibody (dilution ratio 1:60) at 37℃ for 1 hour.
Then cells were stained with the Alexa Fluor®-conjugated Goat
Anti-rabbit IgG secondary antibody (green).
FAQ
1. Q:Are you a factory or trading company?
A: We are a factory, we have rich experience for manufacturing Medical Device more than 10 years.
2.Q:Where is your factory located? How can i visit there?
A: Our factory is located in Jiaxing city, Zhejiang Province, China.
It is about more than 1 hours from Shanghai.
2. 3.Q:Can you make samples?
A: Yes, we can make samples for you.
4.Q:How does your factory do as quality control?
A: Quality is the lift of our factory. We have inspector in
different post, and there are three inspectors before packing to
get best quality products.
5.Q: How is your post market service ?
A: If any damaged rings in the course of carriage, with the
checking it's our mistake, we will take full responsibility to compensate the customers.
6.Q:Can you do OEM for us?
A: Certainly. We also can build the moulding for our new customer.
7.Q:Does the price include freight?
A:No, please choose the express service provider or negotiate with
us.
