Recombinant Mouse Monoclonal antibody SARS-CoV-2 / 2019-nCoV Spike
S2 ECD Protein
Background
The term 'peplomer' is typically used to refer to a grouping of
heterologous proteins on the virus surface that function together.
The spike (S) glycoprotein of coronaviruses is known to be
essential in the binding of the virus to the host cell at the
advent of the infection process. It's been reported that SARS-CoV-2
(COVID-19 coronavirus, 2019-nCoV) can infect the human respiratory
epithelial cells through interaction with the human ACE2 receptor.
The spike protein is a large type I transmembrane protein
containing two subunits, S1 and S2. S1 mainly contains a receptor
binding domain (RBD), which is responsible for recognizing the cell
surface receptor. S2 contains basic elements needed for the
membrane fusion. The S protein plays key parts in the induction of
neutralizing-antibody and T-cell responses, as well as protective
immunity. The main functions for the Spike protein are summarized
as: Mediate receptor binding and membrane fusion; Defines the range
of the hosts and specificity of the virus; Main component to bind
with the neutralizing antibody; Key target for vaccine design; Can
be transmitted between different hosts through gene recombination
or mutation of the receptor binding domain (RBD), leading to a
higher mortality rate.
| Name | Recombinant Mouse Monoclonal antibody SARS-CoV-2 / 2019-nCoV Spike
S2 ECD Protein | Application | Invitro research and manufacture use;
ELISA assay, |
| Formulation | 0.2 μm filtered solution in PBS | Buffer: | 1X PBS buffer |
| Storage: | This antibody can be stored at 2℃-8℃ for one month without
detectable loss of activity. Antibody products are stable for
twelve months from date of receipt when stored at -20℃ to -80℃.
Preservative- Free. Avoid repeated freeze-thaw cycles. | Concentration | ELISA: 1:5000-1:10000 |
| Expiration Date: | 30 days refrigerated at -2-8ºC
and twelve months frozen at -20 ºC
or below from production date.
To be evaluated independently | Feature | High Sensitivity and Specificity |
Specification Data
| Description: | Mouse Monoclonal antibody SARS-CoV-2 / 2019-nCoV Spike S2 ECD
Protein
|
| Clone: | 001 | |
| Source: | mouse (variable region) / human (kappa / IgG1 constant) chimeric
antibody | |
| Immunogen: |
SARS-CoV-2 (2019-nCoV) Spike S2 Protein | |
| Formulation: |
0.2 μm filtered solution in PBS | |
| Purification: | DEAE chromatography | |
| Concentration: | 1.0 mg/mL (OD280 nm, E0.1% = 1.40) |
| Affinity Constant: | Not determined | |
| Buffer: | 0.015M potassium phosphate buffer, 0.85% NaCl, 0.05% NaN3 pH=7.2 |
| Pairing Assays: | N/A | |
| Purity: | >95% by HPLC and SDS-PAGE |
| Application: |
ELISA IHC, FCM, IF, IP et al. applications haven't been validated. |
Chemical stability : Stable under recommended storage conditions.
Possibility of hazardous reactions : No data available.
Conditions to avoid : Repeated freeze thaw cycles.
Incompatible materials : Strong oxidizers.
Hazardous decomposition products : No dangerous decomposition products known.
FAQ
1. Q:Are you a factory or trading company?
A: We are a factory, we have rich experience for manufacturing Medical Device more than 10 years.
2.Q:Where is your factory located? How can i visit there?
A: Our factory is located in Jiaxing city, Zhejiang Province, China.
It is about more than 1 hours from Shanghai.
2. 3.Q:Can you make samples?
A: Yes, we can make samples for you.
4.Q:How does your factory do as quality control?
A: Quality is the lift of our factory. We have inspector in
different post, and there are three inspectors before packing to
get best quality products.
5.Q: How is your post market service ?
A: If any damaged rings in the course of carriage, with the
checking it's our mistake, we will take full responsibility to compensate the customers.
6.Q:Can you do OEM for us?
A: Certainly. We also can build the moulding for our new customer.
7.Q:Does the price include freight?
A:No, please choose the express service provider or negotiate with
us.
