Recombinant 2019-nCoV Mouse Monoclonal Antibody Spike RBD Protein
Background
The term 'peplomer' is typically used to refer to a grouping of
heterologous proteins on the virus surface that function together.
The spike (S) glycoprotein of coronaviruses is known to be
essential in the binding of the virus to the host cell at the
advent of the infection process. It's been reported that SARS-CoV-2
(COVID-19 coronavirus, 2019-nCoV) can infect the human respiratory
epithelial cells through interaction with the human ACE2 receptor.
The spike protein is a large type I transmembrane protein
containing two subunits, S1 and S2. S1 mainly contains a receptor
binding domain (RBD), which is responsible for recognizing the cell
surface receptor. S2 contains basic elements needed for the
membrane fusion. The S protein plays key parts in the induction of
neutralizing-antibody and T-cell responses, as well as protective
immunity. The main functions for the Spike protein are summarized
as: Mediate receptor binding and membrane fusion; Defines the range
of the hosts and specificity of the virus; Main component to bind
with the neutralizing antibody; Key target for vaccine design; Can
be transmitted between different hosts through gene recombination
or mutation of the receptor binding domain (RBD), leading to a
higher mortality rate. This antibody was produced from a hybridoma
resulting from the fusion of a mouse myeloma with B cellsobtained
from a mouse immunized with purified, Recombinant SARS-CoV-2
(2019-nCoV) Spike RBD-mFc Protein. The IgG fraction of the cell
culture supernatant was purified by Protein A affinity
chromatography.
| Name | 2019-nCoV Mouse Monoclonal Antibody Spike RBD Protein
| Application | Invitro research and manufacture use;
membrane assay, ELISA assay, |
| Perseverative | 1 0.02% sodium azide.
| Buffer: | 1X PBS buffer |
| Storage: | Short-term: refrigerated at 2-8 ºC; long term: frozen at or
below -20 ºC. | Concentration | 1.0 mg/mL |
| Expiration Date: | 14 days refrigerated at -2-8ºC
and two years frozen at -20 ºC
or below from production date.
To be evaluated independently | Feature | High Sensitivity and Specificity |
Specification Data
| Description: | Monoclonal Antibody to Acetaminophene |
| Clone: | SARS-CoV-2 (2019-nCoV) Spike RBD Protein | |
| Source: | Mouse IgG2b | |
| Immunogen: |
SARS-CoV-2 (2019-nCoV) Spike S1 Protein | |
| Formulation: |
0.2 μm filtered solution in PBS | |
| Purification: | DEAE chromatography | |
| Concentration: | 1.0 mg/mL (OD280 nm, E0.1% = 1.40) |
| Affinity Constant: | Not determined | |
| Buffer: | 0.015M potassium phosphate buffer, 0.85% NaCl, 0.05% NaN3 pH=7.2 |
| Pairing Assays: | N/A | |
| Purity: | >90% by HPLC and SDS-PAGE |
| Application: |
Neutralization, ELISA |
| Storage: | 2-8o C for 1 year. Store at -200 C for long term. |
The neutralization activity is Measured by microneutralization
assay in vitro. The virus microneutralizaiton (MN) test was
performed on 293T-ACE2 cells infected with SARS-CoV-2 (2019-nCoV)
Spike Pseudovirus under treatment of serial dilutions of
neutralizing antibody. The infection was neutralized by increasing
concentrations of Anti-SARS-CoV-2 Neutralizing Antibody (Catalog#
40592-MM57). Rate of inhibition was determined by comparing the
Relative Light Unit (RLU) of Luciferase reporter in different
antibody concentrations. The IC50 is typically 0.41 µg/mL.
Loaded SARS-CoV-2 (2019-nCoV) Spike RBD-His Recombinant
Protein,Biotinylated on SA Biosensor, can bind SARS-CoV-2
(2019-nCoV) Spike Neutralizing Antibody, Mouse MAb with an affinity
constant of 0.07 nM as determined by Octet RED System.
Loaded SARS-CoV-2 (2019-nCoV) Spike S1-His Recombinant
Protein,Biotinylated on SA Biosensor, can bind SARS-CoV-2
(2019-nCoV) Spike Neutralizing Antibody, Mouse MAb with an affinity
constant of 0.05 nM as determined by Octet RED System
FAQ
1. Q:Are you a factory or trading company?
A: We are a factory, we have rich experience for manufacturing Medical Device more than 10 years.
2.Q:Where is your factory located? How can i visit there?
A: Our factory is located in Jiaxing city, Zhejiang Province, China.
It is about more than 1 hours from Shanghai.
2. 3.Q:Can you make samples?
A: Yes, we can make samples for you.
4.Q:How does your factory do as quality control?
A: Quality is the lift of our factory. We have inspector in
different post, and there are three inspectors before packing to
get best quality products.
5.Q: How is your post market service ?
A: If any damaged rings in the course of carriage, with the
checking it's our mistake, we will take full responsibility to compensate the customers.
6.Q:Can you do OEM for us?
A: Certainly. We also can build the moulding for our new customer.
7.Q:Does the price include freight?
A:No, please choose the express service provider or negotiate with
us.
